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Image Search Results
Journal: Science Advances
Article Title: Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions
doi: 10.1126/sciadv.abb6595
Figure Lengend Snippet: ( A ) Schematic of HTP transfection with a photo perforation transfection system (PTS) was used. ( B ) Representative images showing HTP transfection. Nuclei were stained by DAPI (blue), and the HA-tagged HTP was stained by FITC-avidin (green). Scale bar, 100 um. ( C ) Quantification of transfection efficiency compared with Lip2000. *** P < 0.001 compared with Lip2000. Data are means ± SEM ( n = 3). ( D ) Schematic of DC modification with glycopolymers. ( E ) Representative images showing green fluorescence on the DC cell surface. Nuclei were stained by DAPI (blue) and biotin-labeled poly-(MAG) (pMB) by FITC-avidin (green). ( F ) Representative images showing the modified DCs incubated in complete medium for specified times (1, 3, and 7 days). ( G ) Viability of engineered DC over the 7-day period. Data are means ± SEM ( n = 3). N.D., not determined.
Article Snippet: Primary antibody hemagglutinin (HA) and
Techniques: Transfection, Staining, Avidin-Biotin Assay, Modification, Fluorescence, Labeling, Incubation